General Cell Lines and Cell Cultures
Brainxell Inc Spinal Motor Neurons (Human iPSC-Derived, Male Line, 1M)
Human spinal motor neurons derived from iPSC line WC-30, male. Contents: 1 vial of ≥1x10^6 viable cryopreserved cells. Marker Expression: Spinal Motor Neurons (BX-0100) have high neuronal purity (>80%) and consist of greater than 70% motor neurons. Labeling with the pan-neuronal marker MAP2 (green) and the motor neuron-specific marker FOXP1 (red) highlights the purity of this neuronal product. Function: Spinal Motor Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings. Related diseases: Amyotrophic Lateral Sclerosis (ALS), Spinal muscular atrophy (SMA), & Spinal Cord Injury. Primary neurotransmitter released: Acetylcholine
Eurofins Discoverx PathHunter U2OS BLK Activity Assay, 2 vials. (93-0882C3)
The PathHunter U2OS BLK Activity Assay is a stable, engineered cell line for use in studying small molecule drug candidates targeting the BLK kinase domain. The cell line is engineered to express the catalytic domain from BLK fused to the cytokine receptor binding domain (CRBD) of JAK2. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the GHR-PK. Activation of the GHR-PK induces receptor phosphorylation by the BLK catalytic domain. This leads to SH2-EA recruitment and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Abcam (R)-Baclofen (mM/ml), GABAB agonist, 25MM_ML
MW 213.66 Da, Purity >98%. More active enantiomer of selective GABAB agonist (R,S)-Baclofen (ab120149). Soluble in 1 ml water to give specified mM/ml concentration.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation, 1 vial. (HTS075M)
The ChemiScreen M5 Muscarinic Acetylcholine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of CHRM5 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen µ Opioid Receptor Membrane Preparation, 1 vial. (HTS101M)
The ChemiScreen µ Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRM1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach or to screen for GPCR activity using GTPγS assays. Increased binding affinities or GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
ATCC Bacillus cereus; 6 x 0.3mL
ATCC 10876-MINI-PACK™ consists of 6 ready-to-use vials of ATCC 10876™ frozen in 200 μL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Bioss Progesterone Receptor - Ser294
Progesterone Receptor (Ser294) Primary Antibody Unconjugated Host Species Mouse Target Species Human Clonality Monoclonal Applications WB IHC 608Concentration Lot Dependent Size 100 ul Storage Buffer 10 mM HEPES (pH 7 5) 150 mM NaCl 100 g per ml BSA and 50% glycerol
Eurofins Discoverx PathHunter U2OS ErbB1 Functional Assay, 2 vials. (93-0863C3)
The PathHunter U2OS ErbB1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated ErbB1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged ErbB1 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx cAMP Hunter CHO-K1 DRD5 Gs Cell Line, 2 vials. (95-0094C2)
The cAMP Hunter DRD5 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD5, a naturally Gs coupled GPCR. Upon activation of DRD5, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter NTSR1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0671C3)
The PathHunter NTSR1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of NTSR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged NTSR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting NTSR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mAGTRL1 β-Arrestin Cell Line, 2 vials. (93-0565C2)
The PathHunter mAGTRL1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. apelin-13) based activation of mAGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mAGTRL1 and EA-tagged β-Arrestin-2. Activation of mAGTRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS AXL Functional Assay, 2 vials. (93-1105C3)
The PathHunter U2OS AXL Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated AXL dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged AXL and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter U2OS HTR2C (VNV) β-Arrestin Cell Line, 2 vials. (93-0938C3)
The PathHunter HTR2C(VNV) cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin) based activation of HTR2C(VNV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VNV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VNV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 MRGPRD β-Arrestin Cell Line, 2 vials. (93-0926C2)
The PathHunter MRGPRD cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-alanine) based activation of MRGPRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRD and EA-tagged β-Arrestin-2. Activation of MRGPRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 NPFFR1 β-Arrestin Cell Line, 2 vials. (93-0944C2)
The PathHunter NPFFR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuropeptide FF) based activation of NPFFR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPFFR1 and EA-tagged β-Arrestin-2. Activation of NPFFR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.